A fluorescent sensor based on aggregationinduced emission. The weak emission of aiegens as molecular species and their bright luminescence as nanoscopic aggregates distinguish. Request pdf preparation of biocompatible aggregationinduced emission homopolymeric nanoparticles for cell imaging a series of new homopolymers. Many efforts have been made to develop new photosensitizers for efficient cancer photodynamic therapy. Photostable aie luminogens for specific mitochondrial imaging and its method of manufacturing thereof. Supramolecular aggregationinduced emission aie has become a research hotspot in cell imaging. In order to improve the cellimaging ability, and particularly, to extend the bioapplication of aiegen, human papillomavirus hpv capsid protein l1 was assembled with the complex of dna and aggregationinduced emission fluorogen 9,10distyrylhydrazine dsai, where the viruslike particles vlps of hpv encapsulate the complex via electrostatic interaction. Principles and applications of aggregationinduced emission. Tpeqcs aggregates inside hela cells to achieve ultra longterm cellular. This book explores the aggregationinduced emission aie effect, which has opened new avenues for the development of advanced luminogenic materials in the aggregate or solid state.
Oct 25, 2019 synthesis, solvatochromism, aggregationinduced emission and cell imaging of tetraphenylethenecontaining bodipy derivatives with large stokes shifts. Aggregationinduced emission applications ebook epub. A novel red aggregationinduced emission enhancement aiee chromophore named bcpa bodipy was synthesized based on the construction units 4n,nbis43,6ditertbutylcarbazol9yl phenyl aminobenzaldehyde bcpa and fluorogen. Recent years have witnessed the fast growth of fluorogens with aggregationinduced emission characteristics aiegens in biomedical research. Aggregationinduced emission aie is a novel photophysical phenomenon which offers a new platform applications for researchers to look into the lightemitting processes from luminogen aggregates, from which useful information on structureproperty relationships may be collected and mechanistic insights may be gained. Due to their high biocapacity and fluorescence, they can help researchers to find and mark the location of proteins. The aggregation induced emission aie feature offered silole biolabels the sensitivity 40 to 140fold higher than that of a startoftheart immunoassay using directly fluorescentlabeled. Coassembly of hpv capsid proteins and aggregationinduced.
Gelation process visualized by aggregationinduced emission. Fullrange intracellular ph sensing by an aggregation. We utilize the objectivebased total internal reection microscopy combined with the living cell incubation. Dear colleagues, aggregation induced emission aie is a term introduced by b. Aggregationinduced emission ebook por ben zhong tang. Organic fluorogens with aggregationinduced emission aie characteristics have demonstrated their potential to be ideal candidates for live cell imaging. Applications is the first book to explore the hightech applications of aie luminogens, including technological utilizations of aie materials in the areas of electroluminescence, mechanochromism, chiral recognition, ionic sensing, biomolecule detection, and cell imaging. Photoactivatable aggregationinduced emission probes for lipid dropletsspecific live cell imaging. Dual aggregation induced emission for enhanced fluorescence sensing of furin activity in vitro and in living cells. All of these dha derivatives displayed distinct aggregationinduced emission aie. Applications is the first book to explore the high tech. Activatable cellpenetrating peptide conjugated polymeric nanoparticles with gdchelation and aggregationinduced emission for bimodal. Induced emission homopolymeric nanoparticles for cell imaging chunping ma pcfm lab, dsapm lab, klghei of environment and energy chemistry, state key laboratory of optoelectronic materials and technologies, school of chemistry and chemical engineering, sun yat.
Recent progress of bodipy dyes with aggregationinduced emission. On the one hand, novel bioprobes with superior physicochemical. Aggregationinduced emission probes for cancer theranostics. Aie is a novel photophysical phenomenon, which offers a new platform for researchers to look into the lightemitting processes from luminogen aggregates, from which useful information on structureproperty. Applications is the first book to explore the hightech applicationsof aie luminogens, including technological utilizations of aie materials in the areas of electroluminescence, mechanochromism, chiral recognition, ionic sensing, biomolecule detection, and cell imaging. Aggregationinduced emission on supramolecular coordination. Aggregation induced emission active cytophilic fluorescent bioprobes for longterm cell tracking. Authors contributing to rsc publications journal articles, books or book chapters do. Dual aggregationinduced emission for enhanced fluorescence. Nanoparticles combining enhanced cellular drug delivery with efficient fluorescence detection are important tools for the development of theranostic agents.
Aggregationinduced emission ebook by ben zhong tang. The polymer exhibits unique aggregationinduced emission aie characteristics and can selfassemble to sizetunable particles in dmfwater mixtures. Aggregationinduced emissionfluorophores and applications. Hong fluorescent probes, cell imaging, protein misfolding. The switchable emission manipulated by hydrogen bonding provided solid evidence for the restriction of intramol.
Imaging intracellular anticancer drug delivery by self. Fabrication of aggregationinduced emission based fluorescent. Luminicell live cell tracking nanoparticles sigmaaldrich. Selfassembled gold nanoclusters for bright fluorescence. A novel crosslinked nanoparticle with aggregationinduced. Yuan y, xu s, cheng x, cai x, liu b 2016 bioorthogonal turnon probe based on aggregationinduced emission characteristics for cancer cell imaging and ablation. A novel aggregationinduced emission fluorescent probe for. A phresponsive drug delivery system with an aggregation induced emission feature for cell imaging and intracellular drug delivery.
Twophoton fluorescence microscopic imaging with excitation in the longerwavelength nearinfrared nir region 1100 nm and emission in the niri region 650950 nm is a good choice to realize deeptissue and highresolution imaging. A fluorescent bioprobe comprising one or more fluorogens labeled on biomolecules. Applications is the first book to explore the hightech. The polymer exhibits unique aggregation induced emission aie characteristics and can selfassemble to sizetunable particles in dmfwater mixtures. Aggregationinduced emission by ben zhong tang overdrive.
Synthesis, solvatochromism, aggregationinduced emission and cell imaging of tetraphenylethenecontaining bodipy derivatives with large stokes shifts. Herein, supramolecular assembly with aie effect was constructed in two stages, where adamantane modified tetraphenylethene selfassembly emitted weak fluorescence, and then after adding. Aggregationinduced emission enhancement and living cell. Photo and aromatic stackinginduced green emissive peptidyl nanoparticles for cell imaging and monitoring of nucleic acid delivery. Aggregationinduced emission enhancement and cell imaging. Photodynamic therapy pdt is a noninvasive treatment for selectively killing malignant tumor cells. Photostable fluorescent organic dots with aggregationinduced emission aie dots for. Preparation of biocompatible aggregationinduced emission. Aggregationinduced emission compounds based on 9,10. Fluorescent dyes with aggregationinduced emission provide. Aptamerdecorated selfassembled aggregation induced emission organic dots for cancer cell targeting and imaging. Aggregationinduced emission enhancement and cell imaging of a. Aggregationinduced emission aie is an abnormal phenomenon that is observed with certain organic luminophores fluorescent dyes most organic compounds have planar structures and higher photoemission efficiencies in solution than in the solid state. The discovery of the aie effect opens a new avenue for the.
Photoactivatable aggregationinduced emission probes for lipid. Xiaofan ji, yang li, hu wang, run zhao, guping tang, feihe huang. Aggregationinduced emission aie, which was first introduced by tang et al. Live cell imaging with luminicell tracker nanoparticles. Jun 23, 2016 here we show the entire gelation process of chitosan liohurea aqueous system by aggregation induced emission fluorescent imaging. Aggregationinduced emission aie molecules eliminate the. The emergence of the aggregationinduced emission aie concept. Facile fabrication and cell imaging applications of. The luminogens are nonemissive in organic solution but become highly emissive when aggregated in aqueous solution. Photostable fluorescent organic dots with aggregation. Aggregationinduced emission for highly selective and sensitive fluorescent biosensing and cell imaging liang huang, liming dai department of macromolecular science and engineering, center of advanced science and engineering for carbon case4carbon, case western reserve university, 10900 euclid avenue, cleveland, ohio 44106. Restriction of intramolecular motion is the working mechanism of aggregation. Tang in 2001 to describe situations in which nonemissive or weakly emissive organic molecules exhibit enhanced luminescence when forced into molecular aggregation in the presence of poor solvents or in the solid state.
Sean lim chief editor the skeptical chemist linkedin. Aggregationinduced emission luminogens for biomedical. Methods in molecular biology methods and protocols, vol 991. Aggregationinduced emission aie active fluorescent probes with multiple binding sites toward atp sensing and live cell imaging hengchang ma, a manyi yang, a caili zhang, b yucheng ma, a yanfang qin, a ziqiang lei, a lu chang, a lei lei, a tao wang a and yuan yang a. A novel crosslinked nanoparticle with aggregationinduced emission properties for cancer cell imaging bin li, a tianhong chen, b zhipeng wang, d zhiming guo, d jhair pena, a lintao zeng bc and jinfeng xing a. A novel red aggregationinduced emission enhancement aiee chromophore named bcpabodipy was synthesized based on the construction units 4n. In this work, we have successfully developed ultrabright organic dots with aggregationinduced emission aie dots and demonstrated their capabilities for cellular imaging and cell tracking. Aggregation induced emission of fluorescent bioprobes and methods of using the same. Since the light emission was induced by aggregate formation, professor tang termed the process aggregationinduced emission aie.
A novel crosslinked nanoparticle with aggregationinduced emission properties for cancer cell imaging. Fluorescence emission polymer is a kind of polymer which can absorb light of certain frequency and then give out light. Recently, aggregationinduced emission aie fluorophores have emerged as attractive bioimaging tools due to flexible controllability, negligible toxicity and superior photostability. Conversion of the fluorescent properties depending on the aggregated states can also provide information on the evolution of. Edited by professor tang, who first discovered this phenomenon, this 2volume reference addresses the fundamentals of aggregationinduced emission aie.
Aggregationinduced emission aie fluorophores as imaging. Xiaomei liu and gaolin liang cas key laboratory of soft matter chemistry, department of chemistry, university of science and technology of china, hefei, anhui 230026, china hefei corresponding author. Addition of poor solvents induces the molecules to aggregate, which turns the emission on and boosts their luminescence efficiencies dramatically aggregationinduced emission or aie. Preparation and selfassembly of amphiphilic polymer with. Aggregationinduced emission by tang, ben zhong ebook. Here we show the entire gelation process of chitosan liohurea aqueous system by aggregationinduced emission fluorescent imaging. Luminicell is founded based on the novel technology of aie, which takes the advantage of. Opposite to conventional organic dyes, the aie luminogens are nonluminescent when molecularly dissolved but highly emissive upon aggregation. Currently, a serious problem obstructing the largescale clinical applications of fluorescence technique is the shallow penetration depth. In this work, we report a novel nano photosensitizer, polymeric micelles aiem with aggregation induced emission. In this chapter, we introduce the marriage between aggregation induced emission and supramolecular coordination complexes, in which the two concepts melt into one that enables a new kind of lightemitting metalorganic materials based on discrete metallacycles and metallacages.
Lightenhanced bacterial killing and less toxic cell imaging. The photosensitizer is a necessary component of photodynamic nanomedicine. Aggregationinduced emission aie polymeric micelles for. The polymer nanoparticles can be used for cell imaging, which provides a potential stable fluorescent tool to monitor the distribution of drugs and bioconjugates in living cells. Accompanied by other pseudo in situ investigations, we propose. Aggregation induced emission enhancement and living cell imaging of novel diarylanthracene conjugated dyes c. Herein, we recapitulate the current advances in the application of aie fluorophores to monitor nddss both in vitroand in vivo. Fullrange intracellular ph sensing by an aggregationinduced emissionactive twochannel ratiometric fluorogen sijie chen, yuning hong, yang liu, jianzhao liu, chris w. Fabrication of fluorescent silica nanoparticles with. Recent progress of bodipy dyes with aggregationinduced. An amphiphilic polymer bearing tetraphenylethene tpe moiety was synthesized by convenient reactions. The polymer nanoparticles can be used for cell imaging, which provides a potential stable fluorescent tool to monitor the distribution of drugs.
Fluorescence imaging and multiple therapeutic functions of aggregation induced emission aiebased cancer theranostics. Aggregation induced emission luminogens aiegens for nondoped organic lightemitting diodes han nie, jian huang, zujin zhao, and ben zhong tang chapter 7, pp 173198. Otherwise said, these fluorophores or fluorescent dyes are much more emissive in solution compared to just the solid form, in that the. Ultrabright organic dots with aggregationinduced emission. The discovery of the aie effect opens a new avenue for the development. Densil s1, chang ch2, chen cl2, mathavan a1, ramdass a3, sathish v4, thanasekaran p2, li ws2, rajagopal s5. Article in small 1247 september 2016 with 299 reads how we measure reads. Jan 28, 20 activatable cell penetrating peptide conjugated polymeric nanoparticles with gdchelation and aggregation induced emission for bimodal mr and fluorescence imaging of tumors bin xia, xu yan.
Cationic quaternized chitosan bioconjugates with aggregation. Fluorescence imaging and multiple therapeutic functions of aggregationinduced emission aiebased cancer theranostics. By enabling light emission in the practically useful solid state, aie has the potential to significantly expand the technological applications of luminescent materials. A novel crosslinked nanoparticle with aggregationinduced emission properties for cancer cell imaging bin li, a tianhong chen, b zhipeng wang, d zhiming guo. The photoluminescence pl of t 2 tps and tpe layers adsorbed on the tlc plates can be turned off and on continuously and reversibly by solvent exposure. Here, we demonstrate this concept by a simple, fast, and robust protocol of cationic polymermediated gold nanocluster au ncs selfassembly into nanoparticles nps of ca. Photobleaching resistance of this synthesized nanoparticle is tested and compared with its similar counterpart, which proves its superiority and capability of long term uorescence emission. Photostable fluorescent organic dots with aggregationinduced. Supramolecular hyaluronic assembly with aggregation. The book presents an overview of this rapidly emerging and exciting area of research, inviting scientists to renew their photophysical knowledge and stimulate new developments in the field. Aptamerdecorated selfassembled aggregationinduced emission. Alternative cell tracking methods are therefore desired to attain reliable, stable, and efficient labeling over a long time.
A phresponsive drug delivery system with an aggregationinduced emission feature for cell imaging and intracellular drug delivery. Applications is the first book to explore the hightech applications of aie luminogens, including technological utilizations of aie materials in the areas of electroluminescence, mechanochromism, chiral recognition, ionic. In addition, our recent discovery that aiegens with high brightness and efficient reactive oxygen species generation in the aggregate state further expand their applications in imageguided cancer surgery and therapy. Aggregationinduced emission aie is a novel photophysical. Fluorescent dyes with aggregationinduced emission provide new probes for cancer diagnosis and therapy. Moreover, the acq dyes with highly planar conjugated structure also exhibit small stokes shift and suffer from reabsorption of the dye emission, further reducing their emission efficiency 8. Aggregationinduced emission enhancement of anthracenederived schiff base compounds and their application as a sensor for bovine serum albumin and optical cell imaging. The fluorescent molecules can readily pass through cell membranes, stain only the. Aggregationinduced emission enhancement of anthracene. A novel aggregationinduced emission fluorescent probe for nucleic acid detection and its applications in cell imaging author links open overlay panel xiaowei xu shengyong yan yimin zhou rong huang yuqi chen jiaqi wang xiaocheng weng xiang zhou. Fluorescent bioprobes comprising luminogen formed nanoparticles comprising luminogens with aggregationinduced emission aie properties, which can be used for longterm cell tracking. This is the first time that erythrocytes have been visualized in the brain through a fluorescence.
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